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This website contains data files associated with the study by Mazumder et al (Nucleic Acids Research, 2013) describing the changes that occur in the S. cerevisiae proteome both in terms of levels and broad nuclear-to-cytoplasmic localization in response to the alkylating agent methyl methanesulfonate   (MMS).   The  yeast   GFP   library   (Huh   et   al,   Nature, 2003; http://www.ncbi.nlm.nih.gov/pubmed/14562095) was used to investigate the response of >4000 individual GFP-tagged strains to MMS-induced genotoxis stress. Once responders had been identified, network analysis in terms of physical, genetic and transcription factors interactions, and Gene Ontology analysis, revealed the global cellular processes affected by MMS. Please see our paper for greater details of this study.

The links on the left provided access to the orginal images, the analysis programs and single cell files. The total size of the images is 2 TB. Hence it has been broken up into smaller units and compressed to facilitate easy transfer. The Excel file describes the distributions of the strains in the plates. Please read the "READ_ME" files in each folder first for additional details.

Please contact "aprotim at mit dot edu" should you have any questions or comments.

Also  follow  this  link  to   look  at   some   previous   studies   from     our   laboratory: http://genomicphenotyping.mit.edu



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